Fig. 2. miR-106b targeted ALEX1 in gastric cancer cells. (a) miR-106b mimics and miR-106b inhibitor were used to treat gastric cancer cells, and the expression of ALEX1 was detected. (b) ALEX1 expression level was detected following PLVX-ALEX1 treatment. (c) Luciferase activity of ALEX1 was detected by luciferase reporter assay following treatment with miR-106b mimics or miR-106b inhibitor. **P<0.01. (d) Cell apoptosis was detected following treatment with miR-106b inhibitor and/or PLVX-ALEX1. **P<0.01 versus control. # P<0.05 versus miR-106b inhibitor treatment. (e, f) Detection of EMT related proteins SNAI2, ZEB1, TWIST1, E-cadherin after cells treated with miR-106b mimics and/or PLVX-ALEX1. *P<0.05 versus miR-106b mimics treatment. (g, h) Detection of apoptotic proteins after cells treated with miR-106b inhibitor and/or PLVX-ALEX1. *P<0.05 versus miR-106b inhibitor treatment.